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doi:10.1016/j.mce.2013.09.025
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The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mmol/L butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, United States) and sonicated for 30 s in an ice bath (Ultrasonic bath, Branson, United States)
2013 Traditionally, archaea were considered to be extremophiles, inhabiting ecosystems with very low pH, low oxygen concentration, high temperature or high salt concentration
