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Fluorescence signals were visualized using a fluorescence microscope (Nikon Ts2R) and quantified by flow cytometry (NovoCyte 3000, Agilent, USA)
Then, the well plates were washed with 1 ml of 0.9% NaCl, and the cells were lysed with 1 ml of chloroform:MeOH:H 2 O (1:3:1) and centrifuged at 15,000 g for 5 min at 4 C
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