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For immunofluorescence staining, brain tissues were dissected out, post-fixed by 4% paraformaldehyde (PFA) for 12 h, embedded in paraffin, cut into 5 mm sections and mounted on slides for subsequent staining
This loss results in deficits in synaptic function, plasticity, and clinically presents with neural malformations, hypotonia, developmental delays, delayed/absent speech, autistic-like behavior, and dysmorphic facial features [48]
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However, always listen to your body and rest if youre truly exhausted
