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Chromatographic grade methanol and acetonitrile were obtained from Alltech Scientific (USA) and other analytical regents were obtained from Beijing Chemical Works (China)

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Depending on experimental settings, cells were stimulated with 100 ng/mL VEGF-C, treated with 25 M CQ, 20 mM ammonium chloride (NH4Cl), and 100 M leupeptin or supplemented with 20 mM AC and cultured for 48 h to analyze sprouting
