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Fifth, confounding factors remain unaddresseddietary fiber (a major precursor of SCFAs) is often consumed alongside omega-3s (e.g., in fatty fish), so changes in SCFAs (24) may be driven by fiber rather than omega-3s, a confounder that was ignored in the original analysis (24, 102)
Comparative Analysis: Mechanistic studies demonstrate synergies, such as TGF-/SMAD inhibitors [57, 58] reducing fibrosis more effectively than macrophage modulation alone [59, 60], though M1/M2 shifts offer broader anti-inflammatory benefits in models [60] compared to ROS-targeted antioxidants [61], which excel in oxidative stress but lack pathway specificity

We treated cells with DEG for 6 h and immunoprecipitated the OGDHc-E2 for analysis by LCMS
