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The diagnosis of asthma was based on a history of recurrent episodes of wheezing, chest tight- ness, and reversible airway obstruction previously documented by a physician

The biosynthesis of autophagosomes involves the appearance of a phagophore in the cytoplasm, which then expands, sequestering cytosomal components, before eventually sealing to form a spherical double-membraned vesicle called the autophagosome The newly-formed autophagosome then translocates to and fuses with the lysosome to form an autolysosome

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