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This paper showed that enzymes in the PPP can be transcriptionally activated by NRF2
You wont want to expose your protein of interest or the interacting proteins to 20% ethanol, so after adding your beads to a plastic column youll first rinse the beads out with deionized water (dIH 2 O)
To further confirm an NRF2-dependent effect of DMF, we compared the expression of those ferroptosis-related factors including CBS in sh NRF2 cells

However, other studies have indicated that nutritional stress resulting from Gln deprivation may promote tumor development
