glutathione affinity chromatography protocol Schematic outline of the GST-His two-step purification method GST Tagged Protein and GST
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The concentration of purified At LDOX was measured with a NanoDrop spectrophotometer (Thermo Fisher Scientific) at A 280nm using an extinction coefficient estimated with ProtParam 50
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Furthermore, keloid tissues were cultured in the medium containing 2% FBS, and in situ TUNEL staining was performed (Fig
