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All cell cultures were maintained in a humidified atmosphere with 5% CO 2 at 37 C

Neurons rely heavily on mitochondrial energy production, and in AD, early defects in mitochondrial dynamics, respiratory chain activity, and calcium buffering impair synaptic function and render neurons more vulnerable to stress

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However, in view of the excellent safety profile of B12, even at high doses, supplementation, ideally as part of a more comprehensive nutritional programme, may still be of value when the clinical picture and/or other factors (e.g