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A separate study focused on the NOTO and NOTO -related genes roles in the commitment of mesendoderm progenitor cells towards a notochordal fate by maintaining an expression of the regulatory factors FoxA2 and T-brachyury ( NOTO -eGFP reporter in a human pluripotent stem cell (hPSC) line using CRISPR/Cas9-mediated genome editing that was verified by comparing them to two human NP cell transcriptome datasets (Zhang et al., 2020)

Yes they work synergistically and can be dosed at the same time

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