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353 patients undergoing hemodialysis were receiving over 6 months of antioxidant therapy (600 mg/day of alpha-lipoic acid and 666 IU/day of mixed tocopherols)
For the detection of COLX and NITEGE, antigen retrieval was performed enzymatically with 10 mg/ml Hyaluronidase (Sigma Aldrich, H3884) in MgCl 2 - free PBS, for 40 min at 37 C
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