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Robinson M, McBryant S, Tsukamoto T, Rojas C, Ferraris D, et al

The role of the orphan receptor SF1 in the development and function of the ovary

Based on their increased size and restricted placement, there is an increased possibility that solubility tags will influence the function of the fusion protein

Each wash should also be analyzed to observe any eluted protein, and bait- and prey-free controls should also be used to confirm that there are no bait-prey interactions in the bait lysate and that the prey protein does not bind to the immobilized support.