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Subsequently, samples were analyzed using gas chromatographymass spectrometry, operating in negative chemical ionization mode (Agilent Technologies, CA), with a Triple-Axis Detector, connected to a gas chromatograph (Agilent Technologies, CA)

122,123 By examining the crystal structure of seleno-GPX4, researchers observed the existence of seleninic acid (GPX4-Se-OO-) within the enzymes active site

It undergoes the process of metabolism and the methyl group converted into a carboxyl group