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This includes confirmed low serum vitamin B12 levels, pernicious anaemia, malabsorption states such as coeliac disease, inflammatory bowel disease, or post-gastric or bariatric surgery, unexplained anaemia, neuropathy or neurological symptoms consistent with vitamin B12 deficiency, a long-term vegan diet with symptoms or low levels, or failure of oral vitamin B12 supplementation

In the Neelakantan study, diet-induced obese (DIO) C57BL/6 mice were treated with NNMT inhibitors including 5-Amino-1MQ analogues

doi:10.7759/cureus.1909 Pechlivani N, Ajjan RA

The NHS advises that taking up to 2 mg (2000 micrograms) per day of vitamin B12 in supplements is unlikely to cause harm