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Southern medical journal, 103(3), 265-267

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The 2017 Neelakantan paper in Biochemistry characterised a fluorescent assay for direct real-time monitoring of NNMT enzymatic activity, providing the analytical foundation for inhibitor screening including 5-Amino-1MQ (PMID 28121423)

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The 4.5 mg dose produced 10.8% mean weight loss at 26 weeks

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The lyophilization process preserves molecular stability and allows researchers to reconstitute the compound for controlled in vitro experimentation

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