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For Co-IP/pull-down experiments, pellets obtained from 500 mL expression cultures were resuspended to a calculated OD 600 of 40 in 25 mM TRIS-HCl pH 7, 150 mM KCl, 5% (v/v) glycerol, 10 mM EDTA, and 2% (w/v) n-dodecyl -maltoside (DDM) and incubated for 1 h at 37 C while rotating
J Oral Pathol 10:269275 Bikker FJ, Ligtenberg AJM, Nazmi K, Veerman ECI, Hof WVNT, Bolscher JGM, Poustka A, Nieuw Amerongen AV, Mollenhauer J (2002) Identification of the bacteria-binding peptide domain on salivary agglutinin (gp-340/DMBT1), a member of the scavenger receptor cysteine-rich superfamily
C., Fletcher, J
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