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The recycling reaction was initiated by the addition of reaction buffer (100 mM sodium phosphate, pH 7.4), supplemented with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) (100 M) (Sigma), NADPH (100 M) (Roth), glutathione reductase (1 U/ml) (Sigma), and EDTA (1 mM) (Sigma)

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Revered for its capacity to rejuvenate and safeguard tissues, particularly in the context of remodeling and repair, GHK-Cu is crucial for sustaining healthy skin, accelerating wound closure, encouraging hair growth, and mediating potent anti-inflammatory and antioxidant responses