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For both trees, multiple sequence alignments (MSA) were constructed using ClustalW2, implementing a default Cost Matrix, the Neighbour-Joining (NJ) clustering algorithm, as well as optimized gap penalties

Another study in *Life Sciences* demonstrated significant improvement in inflammatory bowel disease markers within four weeks of treatment (Vukojevic et al., 2018)
Step 3: Drawing and Introducing the Diluent Take your sterile syringe and draw up your chosen volume (e.g., 1mL) of bacteriostatic water