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Another modification of the method is Raman optical activity (ROA) that detects chiral molecules by measuring a small difference in their vibrational Raman scattering intensity when illuminated with right- and left-circularly polarized light (incident circular polarization, ICP) (Zhu et al., 2005)

Drinking and Eating For the first 24 hours, drink two litres of water

Temperature shock can cause precipitation, where the peptide crashes out of solution and forms visible particles that indicate permanent damage