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Description
Amigo, L., & Hernndez-Ledesma, B

Furthermore, MC4R-deficient mice develop severe obesity accompanied by markedly elevated plasma leptin concentrations and leptin resistance, failing to respond to leptins anorectic and cardiovascular effects [58, 59]
Then neurons were washed three times in PBS for 5 min

PMID: 38735588, PMCID: PMC11612925, DOI: 10.1016/j.ypmed.2024.108001