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Using a combination of direct glutathione and precursors can be a balanced approach
The cells were then washed with PBS buffer, lysed with lysate buffer [100 l containing 50 mM PBS, 0.1 mM phenylmethanesulfonyl fluoride (5 l) and Triton X-100 (5 l)], and frozen at 80C for 30 min
This is often less common with liposomal formats because they are gentler on the stomach, but it is still something to monitor
Peptide Sciences understood that early on
