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In keeping with nutritional standards reflected globally in dietary guidelines (National Health & Medical Research Council, 2013) and food regulations (European Food Standards Authority, 2023

85-87 A growing body of evidence indicates that cytokines, especially in combination, may become an important tool in augmenting CD4+ cell populations and CD+8 function in HAART-treated HIV patients

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Fixation was carried out by incubating the cells with 4% paraformaldehyde in PBS containing 0.1% saponin for 45 min at 4 C, followed by washing with PBS supplemented with 0.1% saponin and 1% BSA (SBP)