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Therefore, the values obtained by HPLC would be considered the most accurate, particularly considering that UV/Vis spectroscopic analysis can be highly dependent on the sample concentration, leading to an overestimation when using highly concentrated samples during measurements

Subsequent experiments showed that the dynamic range of InrS was poised to track with the degree of Ni(II)-saturation of a cytosolic-like buffer (Section 6.5.2)
