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Later, the same group, using vesicles containing 37% cholesterol and asymmetric membranes with an SM-rich outer leaflet demonstrated the formation of ordered domains in the inner leaflet containing only PC with two unsaturated fatty acyl groups (PC 18:1/18:1) 87

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Given the very low water content of the solvent-free liquid lipases, it seems plausible that the low threshold absorbance values observed in the ultravioletvisible spectroscopy profiles were associated with limited turnover of the two-step cycle such that a large population of acylated enzymes were present in the solvent-free medium (Fig

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