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Role of cuproptosis in cancer immunotherapy Immune checkpoint blockade (ICB) is a cancer immunotherapy strategy that disrupts inhibitory signaling pathways (e.g., PD-1/PD-L1) to reactivate T-cell-mediated antitumor immunity, effectively overcoming tumor immune evasion and demonstrating clinical success across multiple cancer types

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Most MS studies utilize structural MRI for identifying lesions and determining if they are active based on gadolinium enhancement ( Materials and Methods Overview of Study Written informed consent, approved by the Institutional Review Board of Thomas Jefferson University, was obtained from all subjects and the study was registered on clinicaltrials.gov with the following identifier: NCT03032601

BT20, MDA-MB-231, MDA-MB-468, and BT549 cells were purchased from the American Type Culture Collection (ATCC) and cultured in Dulbeccos Modified Eagle Medium (DMEM), except BT549 which was cultured in RPMI, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin/l-glutamine