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This is consistent with previous work in this area.[7, 5660] Further experiments using N-15 NMR showed that protonation of the pyridine nitrogen promotes proton transfer from O3 to the imine nitrogen when aldimines are formed from aliphatic amines but not from aromatic ones, and detailed the natures of the inter- and intramolecular O-H-N hydrogen bonds.[53, 54] Using model studies as a guide, it was possible to determine the protonation state of the pyridine nitrogen of PLP bound to AAT.[52] In crystalline samples of both the unliganded and maleate-liganded enzyme forms at low pH, the pyridine nitrogen is fully protonated

Preclinical Studies in Diabetic Experimental Models Concerning diabetic retinopathy, in vivo studies in diabetic rats demonstrated that long-term administration of ALA exerts inhibitory effects on the development of diabetic retinopathy through inhibition of the accumulation of oxidatively modified DNA, indicating that ALA supplementation as adjunctive therapy may help prevent vision damage in patients with diabetes [64]