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Given the 100-mV lower redox potential of GSSH relative to GSH (Fig
Purified GST-MFAP2 (full-length) was immobilized on GlutathioneSepharose 4B beads by incubating 20 g of the protein with 30 L of pre-washed beads in binding buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM DTT, and 0.1% Triton X-100) (Beyotime, Shanghai, China) for 1 h at 4 C with gentle rotation
And in the eyes, tiny vessels weaken, light itself becoming clouded through retinopathy
