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Esta prdida es recuperada por la propia sntesis endgena y por la dieta, fundamentalmente procedente de la carne y pescado (tambin suplementos en el caso de consumirlos)
RNA isolation and quantitative RT-PCR gene expression levels The total RNA from the kidney tissue was isolated using the TRIzol reagent (Invitrogen) [27], and the cDNA was synthesized following the manufacturers protocol, using 1 g RNA (Prime ScriptTM RT reagent Kit, Takara)
However, some findings have inherent limitations due to tight sample size, uneven gender distribution, and lack of control groups
