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Description
For example, 10% final concentration of DMSO in the PCR mixture with Phusion decreases primer annealing temperature (i.e., primer melting temperature) by 5.56.0 C (9.910.8 F)
What made a difference this time
However, the use of single or multiple strains remains uncertain due to the low quality of studies currently available, which makes further research necessary [8]
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