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Dimethyl sulfoxide diluted in a .01 M glycine buffer was added to solubilize the cell membranes and the absorbance of reduced MTT in the buffer was quantitated at 590 nm using a plate reader (Biotek Synergy 2, Winooski, VT)
Com base nas fontes disponveis, na poca da sua publicao, o BPC 157 no estava explicitamente listado na lista de substncias proibidas da Agncia Mundial Antidoping (WADA)
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