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Cellular Thermal Shift Assay (CETSA) HEK293T cells cultured in 100 mm 20 mm tissue culture dishes were treated with media containing DMSO or GPX4 activator for 3 h at 37C in a tissue culture incubator (with 5% CO 2 )

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The comorbidity spectrum encompasses epilepsy, anxiety, sensory abnormalities, and other multisystem disorders, necessitating comprehensive strategies targeting GABA receptors, ion channel regulation, and the gut-brain axis ( 4.4 The application of GABAergic drugs in the treatment of ASD 4.4.1 GABA receptor agonists In the clinical treatment of ASD, the application of GABA receptor agonists is becoming increasingly widespread

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