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Neuroscience 145 , 256264 (2007)

In order to modify the transition/processing temperature and particle stability in plasma, phospholipids like 1,2-dipalmitoyl-sn- glycero-3-phosphocholine (DPPC), dimyristoylphosphatidylcholine (DMPC), hydrogenated soy phosphatidylcholine (HSPC), soy phosphatidylcholine (SPC), distearoyl phosphatidylcholine (DSPC), or egg yolk phosphatidylcholine (EYPC) are used in different ratios with cholesterol (Choi), where less Choi in the mixture will result in less stable liposomes in plasma

For Syn 1103 and Syn 1103 -104C constructs, eluted fractions were further purified using a HiTrap Heparin HP column (Cytiva, MA, USA), leveraging cation-exchange conditions due to their higher isoelectric point (pI ~9.5)

Tseng CK, Ho CT, Hsu HS, Lin CH, Li CI, Li TC, et al