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Dimethyl sulfoxide diluted in a .01 M glycine buffer was added to solubilize the cell membranes and the absorbance of reduced MTT in the buffer was quantitated at 590 nm using a plate reader (Biotek Synergy 2, Winooski, VT)

Phase 3: Optimization (ongoing) Optimization involves gradual concentration adjustments based on established tolerance and observed results

Subsequently, to test whether the quality of oocytes is improved at this concentration, the morphology of spindle and the distribution of CGs in oocytes were statistically analyzed at 6h, 12h, and 24h in vitro

Evaluation of anti-inflammatory property of curcumin (diferuloyl methane) in patients with postoperative inflammation