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While melt shift analysis helps identify proteins with biophysical changes, TPCA is a method that more specifically monitors binary protein interactions and protein complex dynamics (75)

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Mitochondria have circular DNA (mitochondrial DNA, mtDNA), and mitochondrial biogenesis (mitobiogenesis) involves the replication, transcription, and translation of mtDNA-encoded genes, the interorganelle transport of phospholipids, and the import of nuclear-encoded proteins into mitochondria through the protein translocation machinery of the outer and inner membranes [14]