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Figure 12 Using stLearn, we visualized the spatial coexpression of FN1 and ITGB1 across all spots, significant spots, and annotated regions, revealing spatially confined ligandreceptor activity ( Figure 12E )

The primary inoculum was prepared by transferring 1 mL of the stock culture to 30 mL of LB medium in a 250 mL flask and culturing at 37 C for 6 h
