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Membranes were washed three times for 5 min with PBST (0.2% Tween 20) and incubated with secondary antibody (dilution, 1:3000) for 1 hour

A study demonstrated the formation of stable DNA/polymer carbon dot (PCD) complexes, where DNA compaction within CDs was confirmed by agarose gel electrophoresis [100]

), so the key is targeted organ cooling (like cold perfusion for transplants or topical cooling of the heart/brain)

[PubMed: 22477279] (Review and commentary on herbal hepatotoxicity discusses kava)
