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The membranes were blocked in 5% not-fat milk or 5% BSA in TBS-T for 60 min at room temperature, incubated with the appropriate primary antibody (+ 4 C, overnight) and then with a secondary antibody conjugated with HRP (60 min at room temperature)

Every batch undergoes LC-MS/MS analysis to confirm structural identity and quantify purity, because peptide integrity directly determines whether the pharmacokinetic and pharmacodynamic properties you're relying on actually manifest in your protocol

[PubMed] [Google Scholar] 105.PCO-02Safety and Pharmacokinetics Trial NCT02637284

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