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First, the remaining cell pellets were washed twice with pre-cooled nanopure water and 150 l 100% MeOH + 0.05% BHT was added

10.1128/MMBR.00038-15
Notably, differences in the timing of disease onset have been observed even among monozygotic twins, and in a case report, one sibling remained symptom-free for up to 13 years after the other had already developed the disease [20]

Hence, CFS is multifactorial disease and may have many and varied genetic contributions (Li et al
