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Description
7 and 8

It plays a central role in fat metabolism and energy production

The cell debris was pelleted by centrifugation at 50,000 g for 60 minutes and the clarified lysate containing the cytoplasmic fraction was loaded onto 10 ml of chitin resin (NEB, USA) packed in a Tricorn 10/100 column (GE Healthcare, USA)

These guidelines and the included recommendations will be reviewed every 2 years
