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154 In particular, lipophilic carriers and some other known carrier systems like cationic amino acids are mainly transported via the transcellular pathway

5.2 Challenges of clinical translation of tumor microbes Tumor-microbe interactions research is experiencing a paradigm shift from correlation to causation, the core of which lies in systematically resolving the molecular mechanisms of key functional bacteria (e.g., Clostridium nucleatum promotes tumor metastasis through activation of the Wnt/-catenin pathway by the FadA adhesin) by means of multi-omics integration technologies (e.g., single-cell sequencing, spatial transcriptomics), and utilizing synthetic biology (e.g., bionanobacterial encapsulation, CRISPRi dynamic regulation) to enhance the colonization efficiency and therapeutic controllability of engineered bacteria in the tumor microenvironment (Li et al., 2023)

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