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The anxiolytic mechanism of Selank involves modulation of GABAergic neurotransmission through allosteric effects on GABA-A receptor sensitivity, distinct from the direct binding site used by benzodiazepines

The permeability of the leak pathway can be better assessed ex vivo by measuring the flux of molecules from the luminal to the basolateral side such as EDTA, mannitol, sucrose, inulin and polyethylene glycols (PEG) or dextran molecules of variable sizes (ranging from 4 up to 20 KDa)

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Ethics declarations Competing interests The authors declare the following competing interests: T.M