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Absorbance was measured at 450 nm using a microplate reader (Thermo Scientific)

Among the seven sirtuins, SIRT1, a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, is the most extensively studied and has emerged as a central regulator of senescence-related liver changes (Wu et al., 2022)

Antioxidants (Basel)

Oral (in diet) administration to mice and rats showed no effects or changes that could be correlated to tribomechanically micronized clinoptilolite-supplementation