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Samples were run on Tris-tricine or Tris-HCl SDS-PAGE gels and transferred to 0.45 m Immobilon-P Polyvinylidene Fluoride (PVDF) (Millipore) for Western blotting using ECL Prime substrate (GE Healthcare)

Curr Genet 59: 91106

189,190 The invasive potential of these cells is driven by upregulated signaling pathways, including L1CAM, ephrin-B2, 191 and epithelialmesenchymal transition (EMT)-associated factors such as twist-related protein 1 (TWIST1), SOX2, and signal transducer and activator of transcription 3 (STAT3)

Louis, MO) containing 20 L of 30% hydrogen peroxide per 11 mL ABTS (preconditioned at RT for 15 minutes) and incubated 30 min
