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However, it is important to note that chelated iron can interact with certain medications, so it is important to talk to your doctor before taking it to develop a safe and effective dosage regimen

Addition of a nonionic detergent, such as 1% Triton X-100, also may be added to the buffer to aid in extraction

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To verify protein expression, a small aliquot of the supernatant was mixed with protein loading buffer, boiled at 100 C for 3 min, and analyzed by SDS-PAGE
