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Affinity purification performed by gravity using TALON resin (Clontech), anion exchange performed using HiTrapQ FF (GE Healthcare), size-exclusion chromatography performed using Superdex 200 10/30 prep grade (GE Healcare) the later two performed using KTA FPLC platform
J Clin Med 10(3):402 Mulhall J et al (2005) Sildenafil citrate response correlates with the nature and the severity of penile vascular insufficiency
C.CeballosG.et al (2010)
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