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Goldstein K, Ghadimi K, Mystakelis H, et al

Strikingly, increasing cellular L2HG levels by knockdown of L2HG dehydrogenase (the only known enzyme that oxidizes L2HG back to -KG) decreased mitochondrial oxygen consumption and lactate production, which indicates that L2HG could inhibit both mitochondrial respiration and glycolysis to blunt their production of NADH and to mitigate attendant reductive stress (72)

According to the manufacturers protocol, a ROS Assay Kit (Beyotime, Shanghai, China, S0033S) with the fluorescent probe DCFHDA was employed

The role of the dorsal root ganglion in the development of neuropathic pain