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The effectiveness of mitochondrial uncoupling activity was assayed by analysing the mitochondrial level of Ucp1, oxygen consumption and mitochondria depolarization
Liberase at 1:65/volume of PBS (Roche, cat # 05401119001) was added, and tissues were incubated at 28C for 35 minutes, pipetting up and down every 5 minutes
Furthermore, HSCs, the resident mesenchymal cells in the liver, are also activated by ROS in viral hepatitis, transforming into myofibroblasts and promoting liver fibrosis and tumor progression [103]
Structural characterization and antioxidant activity of polysaccharide from four auriculariales
