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1000 g solubilized protein extracted from rat striatal tissue was incubated in the presence of anti-D2R antibody (Proteintech Group, rabbit, catalogue# 550841-AP) or control IgG (Merck Millipore, rabbit, catalogue# 12370), together with protein A/G plus agarose (Santa Cruz Biotechnology) overnight at 4 C

Cages were exchanged for new sanitized cages filled with irradiated bedding every 2 weeks
