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Individuals consuming high-beef diets have increased populations of phenol-producing anaerobic Bacteroides compared to individuals on vegetarian diets.8788 Comparisons of western and eastern populations indicated that individuals who consume a typical British and American high-meat diet have a higher ratio of anaerobic to aerobic microflora than persons from Japan, Uganda or India, whose diets are largely vegetarian.89 These findings were confirmed in a study of volunteers who adopted a strictly vegan diet which led to a decrease in serum and urinary phenol and p-cresol concentrations.59 Upon returning to a conventional diet that included the addition of meat and eggs, the volunteers urinary phenol and p-cresol concentrations correspondingly increased.59 Substitution of carbohydrate for protein may not be the only factor responsible for the reduction in phenol levels seen in vegetarians

To induce CRISPRi-mediated gene silencing of metB in Z::metB KD strain, doxycycline (1 mg/ml) was administered to mice via oral route at indicated time points 53

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