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A., Lloyd, E

Vitamin C supplement use may protect against gallstones: an observational study on a randomly selected population

68 Igusa et al

Subsequently, 225 L of substrate solution (100 mM Na phosphate, pH 7.0, 20 mM KCl, 1.0 mg mL 1 o -nitrophenyl--D-galactoside, 2.7 L mL 1 -mercaptoethanol) was added to the permeabilized-cell mixture, and the resulting reaction was incubated at 30 C with shaking
